The 8 well enzyme strips & plates are designed to be used for Enzyme-linked Immunosorbent Assays (ELISA). These are disposable, transparent, polystyrene microplates with a flat bottom that are preassembled in different strip matrixes to accommodate the binding of molecules with hydrophobic and/or hydrophilic properties. They are available in high or medium binding options for ELISA and RIA assays. These plates are manufactured in sterile conditions to meet SBS and ANSI standards for use in a wide range of laboratories.

The ELISpot assay uses a protein secreting cell to capture a target analyte and then detect it using a detection antibody. During the assay, the cells are placed on a plate with a membrane surface coated with a specific capture antibody. This captures the analyte, which then binds to the detection antibody. The captured analyte is then detected by adding a substrate, which precipitates and generates a signal that can be read as visible spots on the membrane surface.

Unlike other plates on the market, these ELISpot plates have a unique coating for enhanced specificity and binding capacity. They are manufactured with a proprietary streptavidin coating that provides significantly increased (up to 5-fold) biotin-binding capacity. This increases the number of binding events, and enables you to conduct assays with higher sample numbers and more complex samples without sacrificing detection sensitivity or obtaining unreadable data. This plate is also available in a clear format that allows for direct imaging of the assay results.

These ELISA plates are preblocked in our Superior Blocking Buffer and ready to use for colorimetric, chemiluminescent or fluorescent assays. They are also fully free of DNase, RNase, human DNA, heavy metals and endotoxins. These plates are manufactured in a Class 100,000 cleanroom and are compatible with all leading automated ELISA machines.

This kit contains 12 Optima DTR 8-well strip plates in a 96-well holder, plus waste trays and a balancing strip. These reusable plates are made of a plastic that is tissue culture treated for cellular adhesion and gamma-irradiated for safety. The trays have a flat, U-bottom design to provide better drainage and are molded with radius edges for smoother wash steps.

To prepare the nitrocellulose membrane, simply add a small amount of ethanol to all the wells on the plate. This will cause the nitrocellulose to instantly become hydrophilic, allowing it to bind the capture antibodies. It is recommended to treat one or two plates at a time to ensure that the PVDF membrane is saturated but not overly saturated. Oversaturation will cause the capture antibody to bind unevenly to the membrane, creating unwanted artifacts in your data. This is a very common problem with uncoated ELISA plates. To avoid this issue, we recommend pre-blocking the nitrocellulose membrane with a blocking buffer before treating it with ethanol. This will prevent the ethanol from becoming too saturated. Once the nitrocellulose is blocked, it can be treated with capture antibody. To do this, add 200 ul of the capture antibody to each well in the plate and incubate it at room temperature for 30 minutes.