Cell culture bottles are mainly used to culture the cells.5 to 500ml bottle size. According to the material can be divided into glass and plastic, the bottom shape of a square, rectangle, triangle, etc., according to the bottle volume of 5 to 500ml for choice. In addition, after the surface is specially modified, the cells can be better cultured, so that according to the area of 25 to 175 cm.
Right-neck culture bottle is ideal for high-volume medium culture, reducing the possibility that the medium will enter the lid when shaking
Torticollis bottle-easier is simpler, improving the use of pipette and spoon
Angle culture bottle improved the ease of using a pipette and reduced the possibility of the medium entering the neck on shaking
Domestic cell culture bottles are generally closed covers, without inner cushions, and are often used in closed culture to ensure their tightness; imported culture bottles have more cover styles: closed, polyester, breathable, cushion, etc. The performance of the airtight cover is the same as that of domestic ones. The polyester cover is often used for open culture. It can ensure the exchange of the gas in the bottle and the gas in the environment. The breathable cap is a 0.2 μ m hydrophobic filter membrane added to the bottle cap to provide sterile gas exchange and reduce the risk of contamination. It is often used in open culture and recommended for CO2 incubator culture, especially for experiments requiring long-term culture. The partition cover is equipped with a pre-cut mouth on the partition pad so that the suction nozzle (or the pipette below 5ml) can be interspersed with the partition to add fluid, suction fluid, or harvest cells, reduce the chance of contamination, and maintain the closed sterile environment of the culture bottle.
The bottleneck of the culture bottle also comes in a variety of styles, including straight neck, torticollis, Angle neck, triangle, and rectangle.
Straight neck: can reduce the culture gene shaking and flow into the bottle cap;
Torticollis: easy to pour into, the pipette or cell scraping is easy to enter the bottle body;
Angle neck: pipette or cell scraping is easy to enter the bottle body, and can reduce the culture gene shaking and flow to the bottle cap;
Triangle: pipette or cell scraping is more likely to reach the bottle Angle, wide bottom increases stability;
Rectangles: The rectangular culture bottle from the bottom to the bottle bottleneck is a slope design, easy to pour, pipette or cell scraping easily into the bottle body, most torticollis bottles have a skirt edge to increase stability. The rectangular bottle of a straight neck and the bottom is the entire bottle surface, saving space and reducing the genetic movement to flow to the cap.