1. Specific determinants of the highly specific PCR response are as follows:

(1)Primers that specifically and correctly bind the template DNA;

(2)Principle of base pairing

(3)TaqDNA Fidelity of the polymerase synthesis reaction;

(4)Specificity and conservation of the target genes

2. High sensitivity

The amount of PCR product increases exponentially, which enlarges the initial template to be tested from pique (pg = 10-12) to microgram (μ G= -6) levels. One target cell can be detected from one million cells; the PCR is sensitive to three RFUs, and three bacteria in bacteriology.

3. Simple and fast

Testable Taq DNA polymerase was used in the PCR reaction. After a disposable addition of the reaction solution, a denaturing annealing extension reaction was performed on the DNA amplification solution and on the water bath, and the amplification reaction was usually completed within 2 – 4 h. Amplified products are usually analyzed by electrophoresis, not necessarily by isotope analysis, and are easily generalized without radioactive contamination.

4. Samsample purity is low