Deep-hole plate is a kind of laboratory plate based on the appearance size of ordinary microhole plate (mainly 96-hole deep-hole plate and 384-hole plate). While keeping the length and width in line with SBS international specifications, the depth of the hole is increased to increase the volume of each hole.

When a new cell line is obtained by a laboratory, it is first cultured and amplified and then frozen in storage. In addition, almost all cells undergo some variation in the process of culture and passage. In order to maintain the consistency of experimental results, cells can no longer be used after dozens of generations of culture. Therefore, cells with few passages should be frozen and cultured for use.

There are four key points to the cell freezing process: cell harvesting, protectant use, freezing rate, and storage environment.

Use of deep hole plate

01 Sample Storage

It can replace the conventional 1.5ml centrifugal tube to store samples, and the storage is neat, saving space, large storage capacity, and can withstand the -80℃ refrigerator. Because of its excellent storage performance, deep hole plate is also called storage board.

02 Sample treatment

High throughput operations on biological samples, such as protein precipitation, liquid extraction, accounting and extraction, can be achieved in combination with the discharge gun, high throughput automatic liquid operation instruments and software. Greatly improve the efficiency of sample processing. It can withstand 121° high temperature and high pressure sterilization.

03 Sample injection operation

The automatic equipment commonly used in various companies can be directly placed in the sample bin of the automatic equipment for sampling. Compared with the traditional sampling, it can not only multiply the number of samples placed in the sample bin, but also realize the direct sampling after the sample is processed on the 96-hole plate, eliminating the tedious work of sampling and placing the sample.

04 Application Direction

Genomic DNA extraction, plasmid DNA extraction and nucleic acid extraction and purification of various samples. High-throughput automated liquid operations, enabling high-throughput operations such as protein precipitation, liquid extraction, animal tissue, bacteria, plants, soil, clinical samples, yeast, etc.

Magnetic rod sleeve: Magnetic rod sleeve is mainly used for nucleic acid extraction on the nucleic acid extraction machine with deep hole plate. In the process of nucleic acid extraction experiment, it can protect the magnetic bar from contamination and prolong the service life of the magnetic bar.

In the process of the experiment, through the up and down movement of the magnetic rod sleeve, the sample in the corresponding magnetic bead method reagent mixing, cracking, combining, washing, elution; Through the coordinated movement of magnetic bar and magnetic bar sleeve, the transfer of magnetic bead and magnetic bead-target compound is realized. Through the separation of magnetic bar and magnetic bar sleeve, the release of magnetic bead and magnetic bead-target compound is realized.