1. First of all, look at the position of the pipette mark, and the scale line, (note: the volume of the pipette volume must be greater than or equal to the required volume).

2. Wash the pipette, wash with tap water or distilled water, to remove it.

3. Observe the arrangement of the pipette scale number, note whether the scale is from top to bottom or from bottom to top, and find the 0 scale value.

4. One holds a suction ball, and the other holds a pipette, (pipette with four fingers, index finger buckle on the upper mouth of the pipette)

5. Take the ear suction ball with the filter paper, wipe the liquid from the outer wall of the pipette; pick up the reagent bottle, ask the reagent bottle and the table of the 45, the pipette head contact the bottle wall and perpendicular to the table, slowly relax the index finger to slow drop zero, pay attention not to make the liquid level drop too fast.

6. When moving to another container, it is also required to hold a hand container, the container, and the table into 45, the pipette head contacts the wall and vertically to the table, relax the index finger to release the volume to be removed.

7. After use, the pipette should be cleaned in time to avoid mixing and contaminating the reagent.