Method of preserving the serum

Serum should be kept at-5℃ to-2O ℃ if stored at 4℃, do not stay for more than one month. If you cannot use one bottle at a time, it is recommended to pack the serum into an appropriate sterilization container and put it back to freezing.

How to thaw the serum without compromising the product quality?

After removing the serum from the freezer, it was dissolved in a 2-8℃ refrigerator and then dissolved at room temperature. However, it must be noted that it must be shaken regularly and evenly during the dissolution process.

How to avoid the production of sediment?

1. When thawing serum, follow the recommended gradual thawing method (-2O ℃ to 4℃ to room temperature). If the temperature changes when the serum is too large (such as-20℃ to 37℃), the experiment shows that sediment is very easy.

2. When thawing the serum, please shake it evenly at any time to match the temperature and composition and reduce the occurrence of precipitation.

3. Do not place the serum at 37℃ for too long. If placed at 37℃ for too long, the serum will become cloudy, and many of the less stable serum components will also be damaged, affecting the quality of the serum.

4. Heat inactivation of serum is very easy to increase the sediment, if not necessary, this step.

5. If heat inactivation of serum is necessary, follow the 56℃, 30 minutes, and shake evenly at any time. The temperature is too high, the time is too long, or uneven shaking will cause the increase of sediment.

After serum thawing is found to have flocculent sediment, how to deal with it?

To remove these flocculent deposits, the serum can be divided into a sterile centrifuge tube, slightly centrifuged at 400g, and the supernatant can then be added to the culture medium and filtered together. However, it is not recommended to remove these flocculent deposits by filtration, because it may block the filter membrane.