With the knowledge of cell culture, we should not only need its basic ingredients, and types but also know some information about the storage methods. Let's explain to you what specific conditions we need for cell culture.

1. Appropriate cell culture medium

A suitable cell culture medium is one of the important conditions for cell growth and proliferation in vitro. The culture medium not only provides cell nutrition and the basic material for cell growth and proliferation but also provides a living environment for cultured cells to grow and reproduce.

2. Serum

Currently, most synthetic media are required for serum addition. The serum is one of the important components of zui in the cell culture medium, which contains various growth factors and other nutrients needed for cell growth.

3. Sterile, nontoxic cell culture environment

The sterile and non-toxic operating environment and culture environment are the first conditions to ensure the successful culture of cells in vitro. Due to the lack of ability to defend against microorganisms and poisons, once contaminated by microorganisms or toxic substances, or their own metabolic substances accumulate, it can lead to cell poisoning and death. Therefore, when cultivating cells in vitro, we must keep the cell survival environment sterile and nontoxic, and remove cell metabolites in time.

4. Constant cell growth temperature

To maintain the vigorous growth of cultured cells, there must be a constant and appropriate temperature.

5. Suitable gas environment

Gas is one of the essential conditions for mammalian cell culture, mainly oxygen and carbon dioxide.